Separation and Quantitative Analysis of Capsaicinoids in Chili Peppers by Reversed-Phase Argentation LC
نویسندگان
چکیده
منابع مشابه
Separation of molecular species of sphingomyelin and ceramide by argentation and reversed-phase HPLC.
A convenient method for the separation of molecular species of complex lipids such as sphingomyelin and ceramide by argentation high performance liquid chromatography (HPLC) is described for the first time. Molecular species separation of these complex lipids was achieved for quantitative analysis and preparative work by the combined use of argentation and reversed-phase HPLC.-Smith, M., P. Mon...
متن کاملDetermination of Polyphenols, Capsaicinoids, and Vitamin C in New Hybrids of Chili Peppers
Six hybrids were subjected to chromatographic analyses by HPLC for the determination of phytochemicals such as capsaicinoid, polyphenol, and vitamin C. The dynamics of ripening of 4 of the hybrids were also characterised. Seven capsaicinoids could be separated and determined; the major compounds were nordihydrocapsaicin, capsaicin, and dihydrocapsaicin, while homocapsaicin and homodihydrocapsai...
متن کاملFast determination of capsaicinoids from peppers by high-performance liquid chromatography using a reversed phase monolithic column
This article reports the development of a rapid and reproducible method of HPLC with fluorescence detection for the determination and quantification of the main capsaicinoids (nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin and homodihydro-capsaicin) present in hot peppers by employing a monolithic column. The type of column employed is a RP-18e (100 mm 4.6 mm) monolithic column...
متن کاملUltrasound-assisted extraction of capsaicinoids from peppers.
The development of a rapid, reproducible and simple method of extraction of the majority capsaicinoids (nordihydrocapsaicin, capsaicin, dihydrocapsaicin, homocapsaicin and homodihydrocapsaicin) present in hot peppers by the employment of ultrasound-assisted extraction is reported. The study has covered four possible solvents for the extraction (acetonitrile, methanol, ethanol and water), the op...
متن کاملReversed-phase separation methods for glycan analysis
Reversed-phase chromatography is a method that is often used for glycan separation. For this, glycans are often derivatized with a hydrophobic tag to achieve retention on hydrophobic stationary phases. The separation and elution order of glycans in reversed-phase chromatography is highly dependent on the hydrophobicity of the tag and the contribution of the glycan itself to the retention. The c...
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ژورنال
عنوان ژورنال: Chromatographia
سال: 2009
ISSN: 0009-5893,1612-1112
DOI: 10.1365/s10337-009-1310-x